Coding

Part:BBa_K1448003:Design

Designed by: yuma kobayashi   Group: iGEM14_KIT-Kyoto   (2014-10-16)


LIMS from Citrus unshiu


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 451
    Illegal XbaI site found at 1410
    Illegal PstI site found at 984
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 451
    Illegal PstI site found at 984
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 451
    Illegal XbaI site found at 1410
    Illegal PstI site found at 984
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 451
    Illegal XbaI site found at 1410
    Illegal PstI site found at 984
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 253


Design Notes

This part has some illegal restriction sites.


Source

this part comes from the coding region of d-limonene synthase isolated from Citrus unshiu.

References